![]() ![]() ![]() The level of statistical significance was set at P 0.30). The significance of the differences between samples was assessed by Student's t-test or Wilcoxon ranked-pairs test after checking for normality. Immediately after the venipuncture all vacuum tubes (each of one additive type) were processed through two different procedures: i) Standard: blood specimens in K(2)EDTA- or sodium citrate- vacuum tubes were gently inverted five times whereas the specimens in tubes with clot activator and gel separator were gently inverted ten times, as recommended by the manufacturer ii) Vigorous mix: all blood specimens were shaken up vigorously during 3-5s independently of the additive type inside the tubes. Blood was collected for routine coagulation, immunochemistry and hematological testing from one hundred volunteers into six vacuum tubes: two 3.6 mL vacuum tubes containing 0.4 mL of buffered sodium citrate (9NC) 0.109 mol/L: 3.2 W/V% two 3.5 mL vacuum tubes with clot activator and gel separator and two 3.0 mL vacuum tubes containing 5.9 mg K(2)EDTA (Terumo Europe, Belgium). ![]() ![]() vigorous) on diagnostic blood specimens collected in vacuum tube systems by venipuncture. To evaluate the effect of tubes mixing (gentle vs. Lima-Oliveira, Gabriel Lippi, Giuseppe Salvagno, Gian Luca Montagnana, Martina Gelati, Matteo Volanski, Waldemar Boritiza, Katia Cristina Picheth, Geraldo Guidi, Gian Cesare Effects of vigorous mixing of blood vacuum tubes on laboratory test results. ![]()
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